Mass Spectrometry Based Method to Increase Throughput for Kinome Analyses Using ATP Probes
نویسندگان
چکیده
منابع مشابه
Mass spectrometry based method to increase throughput for kinome analyses using ATP probes.
Protein kinases play critical roles in many biological and pathological processes, making them important targets for therapeutic drugs. Here, we desired to increase the throughput for kinome-wide profiling. A new workflow coupling ActivX ATP probe (AAP) affinity reagents with isotopic labeling to quantify the relative levels and modification states of kinases in cell lysates is described. We co...
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ژورنال
عنوان ژورنال: Analytical Chemistry
سال: 2013
ISSN: 0003-2700,1520-6882
DOI: 10.1021/ac303478g